Anti-Trypanosoma cruzi ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Intended Use

The ELISA is intended for qualitative detection of specific human IgG antibodies targeting Trypanosoma cruzi (T. cruzi) antigens (1-4). This product is for research use only. Not for use in diagnostic procedures. The performance characteristics of this device have not been established.

Contents of Kit

Warning: Do not use any reagents where damage to the packaging has occurred.
The ELISA contains sufficient reagents for 96 wells. Each kit contains the following reagents:
1. Coated Microtiter Strips: ELISA plate strip holder with 96 (12×8 strips) coated polystyrene microtiter wells. Store at 2-8°C until expiry.
2. Sample Dilution Buffer: One bottle, 25 mL, to be used for preparing sample dilutions. A slight precipitate may form. Mix gently before use. Store at 2-8°C until ready to use or expiry.
3. 100× Conjugate for Chagas: One vial, 150 μL. This contains horseradish peroxidase-labeled anti-human IgG antibody. Mix well prior to use. The 100× Conjugate is added to the Conjugate Diluent before use. Store at 2-8°C until expiry.
4. Conjugate Diluent for Chagas Detect: One bottle, 12 mL, is to be used to dilute the 100× conjugate before adding to the ELISA plate. Stable at 2-8°C until the expiration date.
5. Chagas Negative Control: One vial, 50 μL of heatinactivated serum. The negative control will aid in monitoring the integrity of the kit. Store at 2-8°C until expiry.
6. Chagas Positive Control: One vial, 50 μL of heatinactivated diluted serum. The positive control will aid in monitoring the integrity of the kit. Store at 2-8°C until expiry.
7. Chagas Cut-off Control: One vial, 50 μL of heatinactivated diluted serum. The positive control will aid in monitoring the integrity of the kit. Store at 2-8°C until expiry.
8. 10× Wash Buffer: One bottle, 120 mL of Wash Buffer to be used for all the wash steps of this procedure. Store at 2-8°C until ready to use or expiry.
9. Liquid TMB Substrate: One bottle, 12 mL, ready-to-use. Store at 2-8°C until ready to use or expiry. The substrate
should be kept in a light-protected bottle at all times as provided.
10. Stop Solution: One bottle, 6 mL, to be used to stop the reaction. Store at 2-8°C until ready to use or expiry
NOTE: All reagents and controls must be allowed to reach room temperature (~25°C) and mixed thoroughly by gentle inversion prior to use. Always practice sterile and aseptic techniques at every step. For example, open all reagents in a BSL-2 hood to avoid contamination with airborne bacteria to maintain shelf life.

Storage

2-8°C