Tiamulin ELISA Kit, Quantitative (DEIA036)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
animal tissue
Species Reactivity
Pig, Poultry
Intended Use
This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc).
Contents of Kit
1. Microtiter plate with 96 wells coated with antigen
2. Standard solutions concentrate
3. Spiking standard solution
4. Enzyme conjugate
5. Antibody solution
6. Substrate solution A
7. Substrate solution B
8. Stop solution
9. 20 x concentrated wash solution
10. 2 x extraction solution
Storage
Storage condition: 2-8°C.
Storage period: 12 months
Performance Characteristics
Cross-reactions
Tiamulin: 100%
Lincomycin: < 0.1%
Spectinomycin: < 0.1%
Apramycin: < 0.1%
Precision
Variation coefficient of the ELISA kit is less than 10%.
Detection Limit
Detection limit: 2ppb
Accuracy: 80±15%
Sensitivity
0.3ppb
General Description
Tiamulin is a pleuromutilin antibiotic drug that is used in veterinary medicine particularly for pigs and poultry. Strict MRL has been established due to the potential side effect in human.
HPLC or LC-MS is the common approach to detect tiamulin, and it is limited for the high expense and cost in sample pre-treatment. ELISA is rapid and accurate, which can be used for tiamulin residue detection muscle samples.

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