Tiamulin ELISA Kit

Environmental risk assessment of products requires information on the physico-chemical properties, persistence and ecotoxicity of the product, its constituents and possible metabolic and degradation products. Experimental investigations are usually required to generate this information and consequently risk assessment can be costly and time consuming. One possible approach to minimising the amount of experimental testing is to supplement experimental data with data predicted using models such as quantitative structure-activity relationships (QSARs). Using these models, information can be generated based primarily on the knowledge of the chemical structure of the substance(s) under investigation. In this study predictive models were used to assess the environmental risk of the veterinary medicine, ECONOR(C) which contains the active ingredient valnemulin. Available experimental data on the properties, degradability and ecotoxicity of valnemulin was supplemented with predicted data. Where possible, experimental data was used to validate the predicted approaches and this indicated that the predictions were accurate. Information on usage, properties and degradability was input to fate models to predict environmental concentrations (PECs) of valnemulin in soil, pore water and groundwater. Comparison of PECs with experimental and predicted ecotoxicity data for valnemulin indicated that that even under 'worst case' scenarios the environmental risk posed by valnemulin was low. (C) 2000 Elsevier Science Ltd. All rights reserved.

A rapid method has been developed for the determination of tiamulin (TML) residue in swine tissues by high performance liquid chromatography. The samples were extracted with phosphate buffer-ethyl acetate solution, and then centrifuged by 5000 rpm for 10 min, 0.1% fartrate solution was added in the supernatant obtained with liguid-liquid-liquid extraction, and the organic phase was eveporated. After fats having been removed with hexan, further purification was performed with Oasis HLB solid phase extraction column. The TML residue was determined by HPLC with a mobile phase of acetonitrile-0.0125% acetic acid(42:58, V/V) and UV detection at 210 nm. The average recovery rates of TML in pork and swine liver were in the range of 80.1% - 92.7% when spiked 25 - 500 mu g/kg. The RSD ranged from 2.0% to 13.9%. The detection limits were 5 mu g/kg and 8 mu g/kg for pork and swine liver, respectively.