Turnip Rosette Virus (TRoV) ELISA Kit

Sample

host plants

Detection Method

iELISA

Intended Use

The test can be used to detect TRoV in infected host plants.

Contents of Kit

Coating Antibody: Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate.
Detecting Conjugate: Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate.
ELISA Plate: Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results.
Controls: Positive control and negative control
Buffers: Coating buffer, sample buffer, enzyme conjugate buffer, washing buffer, substrate buffer and PNP tablets.
Instruction: Specific test instructions for each of the three different ELISA Formats.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Turnip Rosette Virus, A virus with isometric particles about 28 nm in diameter. Found rarely in turnip (Brassica rapa) and swede (B. napus) crops in Scotland and transmitted by inoculation of sap and possibly by flea-beetles. It infects very few hosts outside the Cruciferae. In turnip the virus induces necrosis of petioles and veins, with severe dwarfing, leaf twisting and rosetting. In swede it causes vein-banding, rosetting and stunting.