Tobacco Rattle Virus (TRV) ELISA Kit

Sample

host plants

Detection Method

sELISA

Intended Use

The test can be used to detect TRV in infected host plans.

Contents of Kit

1. Coating Antibody: Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate.
2. Detecting Conjugate: Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate.
3. ELISA Plate: Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results.
4. Controls: Positive control (1 vial) and negative control (1 vial).
5. Coating buffer 10×
6. SB1 Buffer
7. PBST Buffe
8. ECB1 Buffer 10×
9. PNP Buffer 5×
11. PNP tablets
12. Tween-20

Storage

PNP tablets at -20°C, under dark. Store other products at 4°C and protect from light as much as possible.
Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling. Prevent direct skin and eye contact with, or ingestion of, product components. Obtain medical attention in case of accidental ingestion of reagent components. All reagent components should be stored at the recommended temperature to assure their full shelf life.

General Description

Tobacco rattle virus (TRV) is a pathogenic plant virus. Over 400 species of plants from 50 families are susceptible to infection. The virus causes the plant disease tobacco rattle in many plants, including many ornamental flowers including Narcissus. It causes the disease corky ringspot in potatoes. The disease manifests in various ways, and signs can include brown rings and arcs on the surface of a potato, and discolored spots on the interior. Nematodes of the family Trichodoridae, the stubby-root nematodes, are vectors of the virus. The nematode species Paratrichodorus minor, for example, introduces the virus when it feeds on the roots of plants. The virus can also be spread on garden tools. It can also be mechanically and seed transmitted.