TPH1 Inhibitor Screening Assay Kit

Intended Use

The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Contents of Kit

1. TPH1, His-tag: 50 μg, -80°C ;
2. TPH Enzyme Solution: 5 ml, -80°C;
3. TPH Reaction Solution: 6 ml, -80°C;
4. TPH Quench Solution: 1 ml, -20°C;
5. Black, 96 Well Plate: Room Temp.

Storage

At least 6 months from date of receipt when stored as directed.

General Description

Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.