TNP [BSA]

In order to achieve the goal of convenient and efficient detection of picric acid in a water environment, based on function-oriented research ideas, we combine luminescence performance with recognition performance, taking into account the application conditions. Urea sites with picric acid selective recognition properties and lanthanide ions with luminescence properties are introduced into the recognition system in a pure water environment and a new 2D luminescent coordination polymer was synthesized. A series of luminescence recognition tests prove that TbL exhibited high sensitivity, good selectivity and fast response towards PA in a pure water environment. Particularly, TbL can not only be simply and quickly regenerated, but also be applied to the detection of PA in river water and tap water, again showing excellent recognition performance. Thus, TbL can be used as an efficient luminescent sensor in the water environment for PA detection. In addition, this work also provides a reference for the future design of novel lanthanide luminescent sensors using function-oriented strategies.

The recent discovery of long-lived plasma cells (LLPCs) in mammals, which provide a constant expression of specific high-affinity antibodies that mediate humoral memory, has caused a dramatic paradigm shift in the study of immunity and vaccine development. In teleost fish, there are few studies regarding the association between LLPCs and antibody production, and the affinity of the antibodies secreted by the LLPCs is poorly understood. In the present study, channel catfish (Ictalurus punctatus) were immunized with trinitrophenylated-keyhole limpet hemocyanin (TNP-KLH) to examine TNP-specific antibody titers, affinities, antibody-secreting cell (ASC) dynamic changes, and especially the affinity of secreted antibodies by LLPCs post-immunization. We demonstrated that TNP-specific LLPCs were generated starting at week 4 post-immunization, achieved a maximal number at week 8, and maintained a comparable level throughout the 18-week post-immunization period, which was correlated with the dynamics of serum antibody titers and affinity maturation in the response. The LLPCs appeared to mostly reside within, or migrate to, the anterior kidney (bone marrow-like tissue in mammals), but a small portion was also located in the spleen and peripheral blood. The antibodies produced by the LLPCs possessed high affinities, indicating that the generation and development of LLPCs were driven by affinity selection in teleosts. Collectively, the results of this study provide insights toward the evolutionary understanding of the affinity-dependent mechanism of LLPC generation and development.