TGF beta3 ELISA Kit

Name: TGF beta3 ELISA Kit
SKU: DEIA-XYA1996
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

COA

SDS

Sample

cultured cells

Species Reactivity

Human, Mouse, Rat

Intended Use

The TGF β3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TGF β3 protein expression profile in cells. The kit can be used for measuring the relative amounts of TGF β3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TGF β3.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-TGF β3 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 Seals

Storage

4°C/6 Months

Citations

Have you cited DEIA-XYA1996 in a publication? Let us know and earn a reward for your research.

Product Name	Cat. No.	Applications	Host Species	Datasheet	Price	Add to Basket

Product Name	Cat. No.	Applications	Host Species	Datasheet	Price	Add to Basket

Certificate of Analysis

Safety Data Sheet

Customer Reviews

Write a review

Autologous adipose-derived stem cell transplantation enhances healing of wound with exposed bone in a rat model

PLOS ONE

Authors: Hamada, Tomo; Matsubara, Hidenori; Yoshida, Yasuhisa; Ugaji, Shuhei; Nomura, Issei; Tsuchiya, Hiroyuki

Abstract

Objectives Soft tissue wounds with exposed bone often require extended healing times and can be associated with severe complications. We describe the ability of artificial dermis with autogenic adipose-derived stem cells (ADSCs) to promote the healing of wounds with exposed bone in a rat model. Methods Adipose tissues harvested from the bilateral inguinal regions of Wistar rats were used as ADSCs. Rats were randomly divided into control and ADSC groups to investigate the efficacy of ADSC transplantation for wound healing (n = 20 per group). Soft tissue defects were created on the heads of the rats and were covered with artificial dermis with or without the seeded ADSCs. Specimens from these rats were evaluated using digital image analysis, histology, immunohistochemistry, cell labeling, and real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). Results The average global wound area was significantly smaller in the ADSC group than in the control group on days 3, 7, and 14 after surgery (p<0.05). After 14 days, the blood vessel density in the wound increased by 1.6-fold in the ADSC group compared with that in the control group (p<0.01). Real-time RT-PCR results showed higher Fgfb and Vegf expression levels at all time points, and higher Tgfb1 and Tgfb3 expression levels until 14 days after surgery in the ADSC group than in the control group (p<0.05). Conclusions In wounds with exposed bone, autogenic ADSCs can promote vascularization and wound healing. Use of this cell source has multiple benefits, including convenient clinical application and lack of ethical concerns.