Sample
serum, plasma, cell culture supernates
Intended Use
For the quantitative determination of human telomerase reverse transcriptase (TERT) concentrations in serum, plasma, cell culture supernates.
Contents of Kit
1. Assay plate (12 x 8 coated Microwells): 1 (96 wells)
2. Standard (Freeze dried): 2
3. Biotin-antibody (100 x concentrate): 1 x 120 μL
4. HRP-avidin (100 x concentrate): 1 x 120 μL
5. Biotin-antibody Diluent: 1 x 15 mL
6. HRP-avidin Diluent: 1 x 15 mL
7. Sample Diluent: 1 x 50 mL
8. Wash Buffer (25 x concentrate): 1 x 20 mL
9. TMB Substrate: 1 x 10 mL
10. Stop Solution: 1 x 10 mL
11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1
Storage
Unopened kit Store at 2-8°C. Do not use the kit beyond the expiration date.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Range
6.25 ng/ml-400 ng/ml
Sensitivity
The minimum detectable dose of human TERT is typically less than 1.56 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Citations
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