Tomato Chlorotic Spot Virus (TCSV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect TCSV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:2430 - 1:7290.

General Description

Tomato chlorotic spot virus (TCSV) is a tospovirus, similar to but distinct from other tospoviruses currently present in Florida, such as Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), and Impatiens necrotic spot virus (INSV). Like these viruses, TCSV is transmitted by thrips (Thysanoptera: Thripidae) and is able to replicate in both the vector and the plant.
TCSV, like other tospoviruses, causes diseases associated with significant losses in overall yield and quality in both agricultural and ornamental crops. TCSV causes extensive necrosis on leaves and fruit, which can significantly reduce yield and fruit quality. As of 2012, TCSV had only been reported to infect tomato plants in Miami-Dade and Lee Counties, causing the onset of severe symptoms that killed newly planted tomatoes, and in an older field, rendered the fruit unmarketable. TCSV is known to cause severe symptoms in plants such as pepper, beans, tobacco, and peanut, and in ornamental crops such as lisianthus. The potential exists for TCSV epidemics in these crops in Florida. The geographic distribution of TCSV in Florida is not known beyond Miami-Dade and Lee Counties. Considering the widespread distribution of thrips, TCSV has the capacity to significantly expand.

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