Rabbit Anti-Human TAF3 (internal) Polyclonal antibody

Deciphering the molecular basis of pluripotency is fundamental to our understanding of development and embryonic stem cell function. Here, we report that TAF3, a TBP-associated core promoter factor, is highly enriched in ES cells. In this context, TAF3 is required for endoderm lineage differentiation and prevents premature specification of neuroectoderm and mesoderm. In addition to its role in the core promoter recognition complex TFIID, genome-wide binding studies reveal that TAF3 localizes to a subset of chromosomal regions bound by CTCF/cohesin that are selectively associated with genes upregulated by TAF3. Notably, CTCF directly recruits TAF3 to promoter distal sites and TAF3-dependent DNA looping is observed between the promoter distal sites and core promoters occupied by TAF3/CTCF/cohesin. Together, our findings support a new role of TAF3 in mediating long-range chromatin regulatory interactions that safeguard the finely-balanced transcriptional programs underlying pluripotency.

A rapid route based on the use of small amounts of polytetrafluoroethylene to chemically activate and sustain the combustion synthesis reaction for the formation of TaC from its elements is successfully exploited in this work. Other than a reaction booster, the polymer is found to play also a role as a carbon source, so that part of graphite can be replaced by Teflon to produce a single phase material. A relevant importance in the activation of the synthesis reaction is provided by the intermediate phase TaF3, whose formation is clearly evidenced, along with that of Ta2C, by combustion front quenching experiments. Additive free TaC products with relative density up to about 98% and grains size less than 5 mu m are finally obtained when combustion synthesized powders are processed for 20 min at 1800 degrees C by Spark Plasma Sintering. A further increase in the sintering temperature to 2050 degrees C and/or the dwell time to 30 min is found to negatively affect product densification. This outcome is mainly ascribed to the significant grains coarsening (above 20 gm) correspondingly observed as well as to other vapor-phase generating events, which could be more easily induced when powders are exposed to higher temperature conditions.