Stefin B ELISA Kit

The problem of cooperative control is especially important in the case of selection of an appropriate mode of operation for a wide class of bio-processes. In classical approach, this can be achieved via SCADA systems used by process operators. However, due to the nonlinear nature of bioprocesses, the operators usually are not able to assess the efficiency of a bioprocess, especially in the presence of self-sustained oscillations (SSO) of the biomass concentration. Hence, they must cooperate with experts who are usually geographically dispersed. This paper presents the solution of the above-stated problems using an additional server application in the layer of supervisory control. The main tasks of the application are to provide the process data (collected by the SCADA system) to a group of experts and allow them to discuss possibilities of enhancing the efficiency of the bioprocess. The taken decisions are then sent to the operator.

Excessive softness is a serious defect of dry cured hams which seems related to high activity of lysosomal cysteine proteinases, such as cathepsin B, in fresh pork muscles a few days after slaughtering. As it has been shown that cathepsin B activity has a moderate heritability in Italian Large White pigs we started a candidate gene approach to identify the gene(s) that affect(s) this parameter. Here, we studied two candidate genes: cathepsin B (CTSB) and cystatin B (CSTB). We amplified and sequenced porcine DNA fragments for these two genes that were used to identify polymorphisms by SSCP and polymerase chain reaction-restriction fragment length polymorphism (,PCR-RFLP) analysis. Four and two alleles were detected at the CTSB and CSTB loci, respectively. Sequencing of the CSTB alleles showed a missense mutation that changes a codon for aspartic acid into a codon for asparagine in exon 3 of the gene. Allele frequencies for the two loci differed among the pig breeds studied (Large White, Landrace, Duroc, Belgian Landrace, Hampshire, Pietrain. Meishan, Cinta Senese, Casertana, Calabrese and Nero di Sicilia). Linkage, somatic cell hybrid panel and radiation hybrid panel analyses assigned CTSB to porcine chromosome (Sscr) 14 and CSTB to Sscr 13. The markers identified at the CTSB and CSTB loci were used in association studies with several traits of economic importance including parameters that may indicate the suitability of pig meat to produce dry-cured hams. Significant associations were observed between CTSB and back-fat thickness and between CSTB and average daily gain. In this study, cathepsin B activity was not associated with the polymorphisms identified at the CTSB and CSTB loci.