Human CTRP-13 ELISA Kit

Name: Human CTRP-13 ELISA Kit
SKU: DEIA-XY2195
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Cell Culture Supernates

Species Reactivity

Human

Intended Use

This Human CTRP13 ELISA Kit contains the necessary components required for the quantitative measurement of recombinant and/or natural human CTRP13 from cell culture supernates, serum and plasma in a sandwich ELISA format.
This immunoassay contains recombinant human CTRP13 and antibodies raised against this protein. Results from this immunoassay have shown to accurately quantify recombinant and natural CTRP13 samples.

Contents of Kit

1. CTRP13 Microplate - 96 well microplate coated with a monoclonal antibody specific for human CTRP13. 1 plate
2. CTRP13 Standard - 500 ng/vial of lyophilized recombinant human CTRP13. 1 vial
3. Detection Antibody Concentrate - 1.2 mL/vial of 10-fold concentrate of lyophilized biotinylated antibody against human CTRP13. 1 vial
4. Positive Control - one vial of lyophilized recombinant human CTRP13. 1 vial
5. Streptavidin-HRP Conjugate - 120 μL/vial of 100-fold concentrated solution of Streptavidin-HRP conjugate. 1 vial
6. Dilution Buffer - 60 mL of buffered solution with preservative. 1 bottle
7. HRP Diluent Solution - 12 mL of buffered solution with preservative. 1 bottle
8. Wash Buffer - 50 mL of 10-fold concentrated buffered surfactant, with preservative. 1 bottle
9. TMB Substrate Solution - 11 mL of TMB substrate solution. 1 bottle
10. Stop Solution - 11 mL of 0.5M HCI. 1 bottle
11. Plate Sealer, 1 piece
12. Plastic Pouch, 1 piece

Storage

##Unopened Kit##: Store at 2-8°C for up to 8 months. For longer storage, unopened Standard, Positive Control and Detection Antibody Concentrate should be stored at -20°C or -70°C. Do not use kit past expiration date.
##Opened/Reconstituted Reagents##: Reconstituted Standard (stock) solution and Detection Antibody concentrated solution SHOULD BE STORED at -20°C or -70°C for up to one month. Streptavidin-HRP Conjugate 200-fold concentrated solution and TMB Substrate Solution can be stored at 2-8°C for up to 8 months (DO NOT FREEZE and PROTECT FROM LIGHT). All other components may be stored at 2-8°C for up to 8 months.
##Microplate Wells##: Return unused strips to the plastic pouch with the desiccant pack. Microplate may be stored for up to 6 months at 2-8°C after opening.

Precision

Intra-assay Precision: 4-6%
Inter-assay Precision: 8-10%

Detection Range

7.8 - 500 ng/mL

Sensitivity

2 ng/mL

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Structures of C1q-like Proteins Reveal Unique Features among the C1q/TNF Superfamily

STRUCTURE

Authors: Ressl, Susanne; Vu, Brandon K.; Vivona, Sandro; Martinelli, David C.; Suedhof, Thomas C.; Brunger, Axel T.

Abstract

C1q-like (C1QL) -1, -2, and -3 proteins are encoded by homologous genes that are highly expressed in brain. C1QLs bind to brain-specific angiogenesis inhibitor 3 (BAI3), an adhesion-type G-protein coupled receptor that may regulate dendritic morphology by organizing actin filaments. To begin to understand the function of C1QLs, we determined high-resolution crystal structures of the globular C1q-domains of C1QL1, C1QL2, and C1QL3. Each structure is a trimer, with each protomer forming a jelly-roll fold consisting of 10 beta strands. Moreover, C1QL trimers may assemble into higher-order oligomers similar to adiponectin and contain four Ca2+-binding sites along the trimeric symmetry axis, as well as additional surface Ca2+-binding sites. Mutation of Ca2+-coordinating residues along the trimeric symmetry axis lowered the Ca2+-binding affinity and protein stability. Our results reveal unique structural features of C1QLs among C1q/TNF superfamily proteins that may be associated with their specific brain functions.

Anatomical and Behavioral Investigation of C1ql3 in the Mouse Suprachiasmatic Nucleus

JOURNAL OF BIOLOGICAL RHYTHMS

Authors: Chew, Kylie S.; Fernandez, Diego C.; Hattar, Samer; Suedhof, Thomas C.; Martinelli, David C.

Abstract

Many biochemical, physiological, and behavioral processes such as glucose metabolism, body temperature, and sleep-wake cycles show regular daily rhythms. These circadian rhythms are adjusted to the environmental light-dark cycle by a central pacemaker located in the suprachiasmatic nucleus (SCN) in order for the processes to occur at appropriate times of day. Here, we investigated the expression and function of a synaptic organizing protein, C1QL3, in the SCN. We found that C1ql3 is robustly expressed in the SCN. C1ql3 knockout mice have a reduced density of excitatory synapses in the SCN. In addition, these mice exhibited less consolidated activity to the active portions of the day and period lengthening following a 15-minute phase-delaying light pulse. These data identify C1QL3 as a signaling molecule that is highly expressed in SCN neurons, where it contributes to the formation and/or maintenance of glutamatergic synapses and plays a role in circadian behaviors, which may include circadian aftereffects.