Schistosoma mansoni IgG ELISA KIT

Sample

serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Schistosoma mansoni IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Schistosoma mansoni in human serum or plasma (citrate)

Contents of Kit

1. Microtiterplate, Schistosoma mansoni Coated Wells (IgG): 12 breakapart 8-well snap-off strips coated with Schistosoma mansoni antigens; in resealable aluminium foil.
2. Sample Diluent: 1 bottle containing 100 ml of buffer for sample dilution; pH 7.2 ± 0.2; coloured yellow; ready to use; white cap.
3. Stop Solution: 1 bottle containing 15 ml sulphuric acid, 0.2 mol/l; ready to use; red cap.
4. Washing Solution (20× conc.): 1 bottle containing 50 ml of a 20-fold concentrated buffer (pH 7.2 ± 0.2) for washing the wells; white cap.
5. Conjugate: 1 bottle containing 20 ml of peroxidase Protein A; coloured blue, ready to use; black cap.
6. TMB Substrate Solution: 1 bottle containing 15 ml 3,3',5,5'-tetramethylbenzidine (TMB); ready to use; yellow cap.
7. Schistosoma mansoni IgG Positive Control: 1 bottle containing 2 ml; coloured yellow; ready to use; red cap.
8. Schistosoma mansoni IgG Cut-off Control: 1 bottle containing 3 ml; coloured yellow; ready to use; green cap.
9. Schistosoma mansoni IgG Negative Control: 1 bottle containing 2 ml; coloured yellow; ready to use; blue cap.

Storage

Store the kit at 2-8°C. The opened reagents are stable up to the expiry date stated on the label when stored at 2-8°C.

Precision