SPR Knockout Cell Lysate

Name: SPR Knockout Cell Lysate
SKU: DAG-KO289
Rating: 5 (1 reviews)

SPR Knockout Cell Lysate from HeLa cell line for anti-SPR antibody specificity validation.

COA

SDS

Datasheet

Summary

Product Overview

HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The SPR knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.

Nature

Cell Lysate

Alternative Names

SPR; sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase); SDR38C1; sepiapterin reductase; short chain dehydrogenase/reductase family 38C, member 1;

Application Notes

Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Dilution

Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.

Format

Lyophilized

Concentration

The protein concentration was determined with BCA assay.

Buffer

RIPA buffer

Preservative

None

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Citations

Have you cited DAG-KO289 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Sensitivity enhancement of SPR biosensors employing heterostructure of PtSe2 and 2D materials

OPTICAL MATERIALS

Authors: Rahman, Md Mahabubur; Rana, Md Masud; Rahman, Md Saifur; Anower, M. S.; Mollah, Md Aslam; Paul, Alok Kumar

Abstract

In this paper, two exclusive surface plasmon resonance (SPR) biosensors are proposed numerically utilizing the newly emerged transition metal dichalcogenides (e.g. PtSe2) composed with 2D materials (e.g. Graphene, MoS2 and WS2) onto the traditional SPR biosensors. The performance of the sensors is investigated using the technique of attenuated total reflection (ATR) in terms of sensitivity, figure of merit (FOM) and quality factor (QF). Among those sensors, it has been noticed that the Ag/PtSe2/WS2 and Au/PtSe2/WS2 structures, respectively, show very high sensitivity of 194 deg/RIU and 187 deg/RIU which are 1.67 and 1.37 times higher than that of the conventional silver (Ag) and gold (Au) based sensors, respectively, with comparable FOM and QF. The thickness of Ag/Au is considered 50 nm and the number of layers of WS2 is adjusted to 4 and 2 for Ag and Au based sensors, respectively. In both cases, an optimized thickness of 2 nm is used for PtSe2. Such impressive performances of the proposed biosensors will pave a way for their use in future medical diagnostics and biomedical detection applications.

Construction of fixed Z-scheme Ag vertical bar AgBr/Ag/TiO2 photocatalyst composite film for malachite green degradation with simultaneous hydrogen production

JOURNAL OF POWER SOURCES

Authors: Liu, Jize; Liu, Zhiyu; Piao, Congcong; Li, Shuguang; Tang, Jianhe; Fang, Dawei; Zhang, Zhaohong; Wang, Jun

Abstract

A novel fixed Z-scheme Ag vertical bar AgBr/Ag/TiO2 photocatalyst composite film is prepared by continuous ion layer adsorption and sol-gel spin coating methods. Meanwhile, an immobilized reactor that can realize the separate collection of H-2 and CO2 is also designed. The activity of the samples is evaluated through MG photocatalytic degradation with simultaneous hydrogen production. Some influence factors, AgBr film layer number, Ag foil thickness and TiO2 film layer number, are investigated on the activity of fixed Z-scheme Ag vertical bar AgBr/Ag/TiO2 photocatalyst composite film. The contribution of Ag SPR effect is also researched. The effects of irradiation time, MG concentration and used times are examined for searching optimum experiment conditions. A possible mechanism on MG photocatalytic degradation with simultaneous hydrogen production is proposed. It is wished that this work can provide a new idea in preparation of immobilized Z-scheme photocatalysts for efficiently degrading organic pollutants with simultaneous H-2 production.