SMURF2 Knockout Cell Lysate (DAG-KO351)

SMURF2 Knockout Cell Lysate from HeLa cell line for anti-SMURF2 antibody specificity validation.

Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The SMURF2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Nature
Cell Lysate
Alternative Names
SMURF2; SMAD specific E3 ubiquitin protein ligase 2; E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Procedure
None
Format
Lyophilized
Concentration
The protein concentration was determined with BCA assay.
Size
100 ug
Buffer
RIPA buffer
Preservative
None
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.

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References


Subramaniam, V; Li, H; et al. The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase. BRITISH JOURNAL OF CANCER 89:1538-1544(2003).
Connor, MK; Azmi, PB; et al. Molecular characterization of ring finger protein 11. MOLECULAR CANCER RESEARCH 3:453-461(2005).

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