Rat Pulmonary Surfactant Associated Protein B ELISA Kit

Name: Rat Pulmonary Surfactant Associated Protein B ELISA Kit
SKU: DEIA-BJ2273
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Species Reactivity

Rat

Detection Method

Competitive ELISA

Intended Use

Rat Pulmonary Surfactant Associated Protein B ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Pulmonary Surfactant Associated Protein B. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL

Storage

All components of this kit are stable at 2-8°C until the kit's expiration date.

Detection Range

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Citations

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Spatial and temporal expression of SP-B and TGF-β1 in hyperoxia-induced neonatal rat lung injury

Dongyun Liu, Yingzi Liu, Liping Dou, Mengya Sun, Hong Jiang, Mingji Yi

Int J Clin Exp Pathol2018 JanPubMed ID: 31938105Read Article

Applications: ELISA
Reactive species: Rat
"Abstract: Objective: Bronchopulmonary dysplasia (BPD) is a severe complication of extreme prematurity that can be caused by hyperoxia inhalation. SP-B and TGF-β have been reported to be implicated in the development of lung. This study aimed to reveal the spatial and temporal expression patterns of these two factors in an animal model of BPD. Methods: Newborn Sprague-Dawley (SD) rats were subjected to hyperoxia conditions to establish an animal model of BPD. The levels of SP-B, TGF-β, MDA and TAOC, as well as the activations of MAPK and PI3K/AKT pathways in lung tissues were monitored during newborn rats prolonged exposure to hyperoxia. Results: We found that hyperoxia exposure significantly induced body weight loss of SD rats. H&E staining for morphometric analyses revealed that hyperoxia arrested alveolar development or loss of alveoli, with fewer and dysmorphic capillaries. mRNA and protein levels of SP-B and TGF-β were high expressed in hyperoxic lung tissues. The concentrations of SP-B and TGF-β in bronchoalveolar lavage fluid were also increased. All these increases begin at the 3th day of hyperoxia exposure. MDA content was increased while TAOC content was decreased in response to hyperoxia. Furthermore, hyperoxia activated p38, and deactivated PI3K and AKT expression. Conclusion: Our research demonstrated that SP-B and TGF-β1 were highly expressed in three levels: mRNA and protein levels in lung tissues, and the release of SP-B and TGF-β1 in bronchoalveolar lavage fluid, beginning at the 3th day of hyperoxia exposure."
Article snippet: SP-B and TGF-β1 concentrations in bronchoalveolar lavage fluids were quantified by using their correspondingly commercial ELISA kits (CD Creative Diagnostics, NY, USA) according to the manufacturer’s prtocol.