The Mouse Semaphorin 4D/CD100 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Semaphorin 4D in serum, plasma and cell culture supernatants.
Contents of Kit
No.
Components
Size
Storage Conditions
1
Mouse Semaphorin 4D Microplate
96T
See Datasheet
2
Mouse Semaphorin 4D Standard Protein
2 vials
See Datasheet
3
Mouse Semaphorin 4D Detection Antibody
2 vials
See Datasheet
4
Wash Buffer (20×)
25 ml
See Datasheet
5
HRP-Streptavidin (200×)
200 μl
See Datasheet
6
TMB One-Step Substrate Reagent
12 ml
See Datasheet
7
Stop Solution
8 ml
See Datasheet
8
Assay Diluent B (5×)
15 ml
See Datasheet
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see 'Reagents And Materials Provided'.
Detection Range
23 pg/ml - 5000 pg/ml
Sensitivity
The minimum detectable dose of Mouse Semaphorin 4D was determined to be 23 pg/ml. Minimum detectable dose is defined as the analyte concentration resulting in an absorbance that is 2 standard deviations higher than that of the blank (diluent buffer).
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