Aspergillus Restrictus Restrictocin (DAG4642)

Aspergillus Restrictus Restrictocin, natural protein from Aspergillus

Product Overview
Aspergillus restrictus Restrictocin
Nature
Native
Tag/Conjugate
Unconjugated
Procedure
None
Format
freeze dried
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Restrictocin is a 149 amino acid ribonucleolytic toxin produced by the fungus Aspergillus. specifically cleaves. It is a member of a group of fungal ribosome inactivating proteins that cleave a single phosphodiester bond within 28S rRNA, which results in a potent inhibition of protein synthesis in eukaryotic cells. Fusion proteins between Restrictocin and monoclonal antibodies have been used as an immunotoxin directed against human carcinoma cells.
Keywords
Restrictocin; Aspergillus restrictus Restrictocin; Aspergillus restrictus; Aspergillus

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References


EFFECTS OF LEADER SEQUENCES UPON THE HETEROLOGOUS EXPRESSION OF RESTRICTOCIN IN ASPERGILLUS-NIDULANS AND ASPERGILLUS-NIGER

CANADIAN JOURNAL OF MICROBIOLOGY

Authors: BRANDHORST, T; KENEALY, WR

The effects of altered leader sequences on the secretion and localization of restrictocin expression in Aspergillus nidulans and Aspe,rguillus niger were investigated. The region encoding the leader sequence of the Asperguillus restrictus restrictocin (res) gene was altered and variants were expressed under the glucoamylase (glaA) promoter in A. nidulans and A. niger. The entire restrictocin leader sequence was replaced by the glaA leader sequence in one variant. In another, the signal sequence of restrictocin was replaced with the glaA signal, leaving a hybrid with the putative restrictocin pro region in place of the glaA pro region. The putative pro region was deleted from the restrictocin leader of a third variant. Toxic effects, such as reduced transcript levels and cellular lysis, were minimal when restrictocin was expressed with the native leader sequence, but became more pronounced as the leader sequence was varied. These toxic effects were inversely proportional to the level of restrictocin secreted. In all transformed strains, restrictocin secretion appeared at the periphery of colonies and was observed to occur at the tips of hyphae. Localization of restrictocin to differentiated structures (conidiophores), as occurs in A. restrictus, was observed only in transformed strains containing the complete restrictocin leader sequence.

Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed

TOXICON

Authors: Martinez-Ruiz, A; Kao, R; Davies, J; del Pozo, AM

alpha-Sarcin, restrictocin and mitogillin are the best known members of the family of fungal ribotoxins. In recent years, new members of this family have been discovered and characterised. In this work, we study the occurrence of ribotoxins among different species of fungi. The presence of ribotoxins has been identified in some new species by means of genetic studies, as well as expression and activity assays. The ribotoxin genes have been partially sequenced, and demonstrate a high degree of similarity. These studies demonstrate that these toxins are more widespread than previously considered. This is surprising, considering the ribotoxins are such specific and potent toxins, of unknown biological function. These studies confirm the hypothesis that these proteins are naturally engineered toxins derived from ribonucleases of broad substrate specificity. (C) 1999 Elsevier Science Ltd. All rights reserved.

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