Product Overview
Recombinant Human Tissue Transglutaminase (tTG),C-terminal. Contains a Strep-Tag. Was expressed in HEK293-EBNA cell line
Tag/Conjugate
Unconjugated
Format
Purified, Lyophilized. Reconstitute with 100ul double distilled water.
Concentration
1 mg/ml (prior to lyophilization)
Buffer
Lyophilized from50mM Tris-HCl, pH 7.5 containing 1mM EDTA, 1mM HABA and 1mM PMSF
Storage
2-8°C short term, -20°C long term
Introduction
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Description
Tissuetransglutaminase (abbreviated as TG2 or tTG) is an enzyme (EC 2.3.2.13) ofthe transglutaminase family. Like other transglutaminases, it crosslinksproteins between an ε-amino group of a lysine residue and a γ-carboxamidegroup of glutamine residue, creating an inter- or intramolecular bond that ishighly resistant to proteolysis (protein degradation). It is particularlynotable for being the autoantigen in coeliac disease, but is also known toplay a role in apoptosis, cellular differentiation, and matrix stabilisation.
Keywords
tTG; Tissue Transglutaminase; TGM2; Celiac disease
Citations
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