HIV type 1 Envelope protein [His], recombinant protein from E. coli
HIV-1 envelope His Tag Recombinant-is an E.coli-derived 74.18 kDa protein that contains immunodominant regions of gp120 C-terminus and gp41 N-terminus of the HIV1 and having 6X His tag protein on the N-terminus.
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Sterile filtered colorless clear solution.
20mM sodium carbonate, pH-9.6 and 0.02% sodium azide.
2-8°C short term, -20°C long term
HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids.Immunodeficiency develops more slowly with HIV-2.HIV-2 is less infectious in the early stages of the virus than with HIV-1.The infectiousness of HIV-2 increases as the virus progresses.Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion.HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.
HIV-2 Envelope 201 Antigen; Retroviridae; Lentivirus; Human immunodeficiency virus 2