HAV P3C [GST]

Product Overview

Recombinant HAV P3C immunodominant region. Contains a GST fusion partner, was expressed in E. coli. Immunoreactive with HAV positive sera.

Nature

Recombinant

Tag/Conjugate

GST

Purity

> 90% pure (10% PAGE coomassie staining). Inclusion Bodies

Format

Purified, Liquid

Concentration

1 mg/ml

Buffer

10mM Carbonate-bicarbonate buffer, pH 9.6, 0.1% SDS containing 50% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Hepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase.

Keywords

HAV P3C; HAV; P3C; Hepatitis A Virus P3C; Hepatitis A Virus; Picornaviridae; Hepatovirus