E. coli Nth (aa 1 - 211) [His], recombinant protein from E. coli
Escherichia coli nth (NP_416150, 1 a.a. - 211 a.a.) full-length recombinant protein with His tag expressed inEscherichia coli .
In 20 mM Tris-HCl, 100 mM NaCl, pH 8.0 (40% glycerol, 1 mM DTT, 0.1 mM PMSF)
2-8°C short term, -20°C long term
Nth is specific for damaged pyrimidine sites, particularly Thy. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction.
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.