E. coli Nth [His] (DAG1749)

E. coli Nth (aa 1 - 211) [His], recombinant protein from E. coli Datasheet

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Product Overview
Escherichia coli nth (NP_416150, 1 a.a. - 211 a.a.) full-length recombinant protein with His tag expressed inEscherichia coli .
Nature
Recombinant
Tag/Conjugate
His
Purity
Conventional Chromatography
Format
Liquid
Concentration
0.5 mg/ml
Buffer
In 20 mM Tris-HCl, 100 mM NaCl, pH 8.0 (40% glycerol, 1 mM DTT, 0.1 mM PMSF)
Storage
2-8°C short term, -20°C long term
Introduction
Nth is specific for damaged pyrimidine sites, particularly Thy. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction.
Antigen Description
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

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