C. Trachomatis Outer Membrane Protein [His]

Name: C. Trachomatis Outer Membrane Protein [His]
SKU: DAG558
Rating: 5 (1 reviews)

C. Trachomatis Outer Membrane Protein [His], recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Product Overview

Recombinant Chlamydia trachomatis major outer membrane protein, contains 6-Histidine epitope tag, immunoreactivity tested with polyclonal antibodies against C. trachomatis strain L2-434 and with C. trachomatis infected human serum, was expressed in E. col

Nature

Recombinant

Tag/Conjugate

His

Purity

> 90% pure (SDS-PAGE). Metal chelate affinity chromatography

Format

Purified, Liquid

Concentration

2.5 mg/ml (Bradford)

Buffer

6M urea, 20mM Tris-HCl, pH 7.4, 0.5M Sodium chloride, 50mM imidazole, 5mM 2-mercaptoethanol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Chlamydia trachomatis, an obligate intracellular human pathogen, is one of four bacterial species in the genus Chlamydia. C. trachomatis is a gram-negative bacterium, therefore its cell wall components retain the counter-stain safranin and appear pink under a light microscope. It can appear as either coccoid or rod shape.

Antigen Description

A membrane protein is a protein molecule that is attached to, or associated with the membrane of a cell or an organelle. More than half of all proteins interact with membranes.

Keywords

Major Outer Membrane Protein; MOMP; omp1; omp1F; Omp1L1; ompIL2; C. trachomatis MOMP; Chlamydia trachomatis MOMP

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

A pilot comparative study of the Chlamydia trachomatis Pgp3 antibody ELISA and two C. Trachomatis-specific MOMP peptide assays on sera from young people in the community

HIV MEDICINE

Authors: Horner, P.; Wills, G.; Pebody, R.; Brown, D.; Broadbent, A.; Colgan, A.; Parker, D.; McClure, M.

Abstract

Characterization of the systemic disease and ocular signs induced by experimental infection with Chlamydia psittaci in cats

VETERINARY MICROBIOLOGY

Authors: TerWee, J; Sabara, M; Kokjohn, K; Sandbulte, J; Frenchick, P; Dreier, KJ

Abstract

In addition to the commonly reported ocular signs, Chlamydia psittaci infection of kittens resulted in fever, lethargy, lameness and reduction in weight gain following ocular instillation of virulent organisms. The appearance of these systemic signs was late with respect to the appearance of ocular symptoms and occurred simultaneously with increasing levels of chlamydia-specific IgG. Measurement of acute phase reactants and IL-6 in plasma indicated that both became elevated concurrent with or slightly after the appearance of fever and remained elevated after the fever began to resolve. Preliminary data also indicated that infectious C. psittaci was present in the blood stream during this time period. The results of ocular instillation of three different levels of C. psittaci (10(3.8), 10(2.8) and 10(1.5) TCID50) indicated that the frequency of infection and the severity of ocular signs were diminished in the group receiving the lowest dose, However, the magnitude of systemic disease was similar in all animals which exhibited clinical signs, irrespective of the dose administered. The immune response to infection included elementary body (EB)-specific lymphocyte proliferation as well as the development of EB-specific IgG and IgM antibodies. The predominant antibody response was to a 45 kDa protein, the major outer membrane protein (MOMP), lipopolysaccharide (LPS), a 58 kDa doublet and 32 and 16-19 kDa proteins. (C) 1998 Elsevier Science B.V.