Detection Method
Sandwich ELISA
Intended Use
The Ras Activation ELISA KIT is designed to detect the presence of activated Ras.
Contents of Kit
1. Glutathione-Coated Plate, 96-well strip format
2. Anti-Ras Detection Antibody
3. Mg2+ Lysis/Wash Buffer, 5X
4. 20% Tween 20
5. 10% BSA in TBS (Blocking Buffer)
6. 20X TBS Wash
7. Gt x Ms, HRP
8. Chemiluminescent Reaction Buffer
9. Chemiluminescent Detection Reagent
Storage
Kit components arrive on dry ice, and must immediately be stored at the temperatures specified below. Kit components are stable for 3 months from date of shipment when stored as directed. For more detailed information, please download the following document on our website.
Citations
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Fornaro, L; Lonardi, S; et al. FOLFOXIRI in combination with panitumumab as first-line treatment in quadruple wild-type (KRAS, NRAS, HRAS, BRAF) metastatic colorectal cancer patients: a phase II trial by the Gruppo Oncologico Nord Ovest (GONO). ANNALS OF ONCOLOGY 24:2062-2067(2013).
Rampias, T; Giagini, A; et al. RAS/PI3K Crosstalk and Cetuximab Resistance in Head and Neck Squamous Cell Carcinoma. CLINICAL CANCER RESEARCH 20:2933-2946(2014).