Species Reactivity
Human, Mouse
Intended Use
The P70 S6 Kinase β Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor P70 S6 Kinase β protein expression profile in cells. The kit can be used for measuring the relative amounts of P70 S6 Kinase β in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on P70 S6 Kinase β.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-P70 S6 Kinase β Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 Seals
Citations
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Xiong, YY; Yepuri, G; et al. ARG2 impairs endothelial autophagy through regulation of MTOR and PRKAA/AMPK signaling in advanced atherosclerosis. AUTOPHAGY 10:2223-2238(2014).
Sharma, A; Janocha, AJ; et al. Targeting mTORC1-Mediated Metabolic Addiction Overcomes Fludarabine Resistance in Malignant B Cells. MOLECULAR CANCER RESEARCH 12:1205-1215(2014).