Raspberry bushy dwarf virus (RBDV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect RBDV in infected host plans.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant leaf tissues diluted at1:2430 - 1:7190.

General Description

Raspberry bushy dwarf virus (RBDV) is a pollen-transmitted virus that has been reported from eastern and western Europe, Scandinavia, Russia, North and South America, Australasia and South Africa, and probably occurs wherever susceptible varieties are grown. In the field the virus infects both red raspberry and black raspberry, although, symptoms vary depending on the cultivar and some cultivars are immune to the virus. RBDV is associated with a range of symptoms including yellowing of the leaf, reductions in cane height and diameter, and decreases in fruit size and yield. In addition, RBDV infection can interfere with the correct development of drupelets in the berry resulting in the phenomenon of crumbly fruit, seriously reducing fruit quality. Severe stunting of the plant, referred to as bushy dwarf disease, only occurs when the plant is infected with both RBDV and a second virus, most likely Black raspberry necrosis virus (BRNV). Indeed, all the symptoms associated with RBDV are greatly enhanced when additional viruses are also present.

Standard Curve