Pentachlorophenol ELISA Kit

Sample

Honey, milk, juice, and rice

Detection Method

cELISA

Intended Use

This kit can be used in quantitative and qualitative analysis of Pentachlorophenol residue in honey, milk, juice, and rice.

Contents of Kit

1. Microtiter plate with 96 wells coated with antigen
2. Standard solutions (5 bottles×0.8ml/tube) 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 8.1ppb
3. Spiking standard solution (0.8ml/tube) 100ppb
4. Pentachlorophenol Antibody#1: 11mL
5. 100× HRP-Conjugated Antibody#2: 0.25mL
6. Antibody#2 Diluent: 20mL
7. 20× Wash solution: 28mL
8. TMB Substrate solution: 12mL
6. Stop solution: 14mL

Storage

Storage condition: 2-8°C
Storage period: 12 months

Precision

C.V. of the ELISA kit: less than 10%

Detection Limit

Milk/Juice/water: 1ppb
Honey: 1ppb
Rice/produce/processed food: 1ppb

Sensitivity

Overall Sensitivity: 0.2ppb

General Description

Pentachlorophenol belongs to the class of organochlorine pesticides and can be used as an insecticide, antibacterial agent, fungicide, algaecide, and preservative. Pentachlorophenol continues to be used, frequently in combination with other pesticides and can easily accumulate in animals and can enter the human body through the food chain. Pentachlorophenol causes harmful effects on the liver, kidneys, blood, lungs, nervous system, immune system, gastrointestinal tract and is a probable human carcinogen.
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.

Standard Curve

1. Calculating the Percentage absorbance
The mean values of the absorbance values obtained from the standards and the samples are divided by the absorbance value of the first standard (zero standard) and multiplied by 100%.
Absorbance (%) = B / B₀ *100
B = the mean absorbance value of each standard or each sample
B₀ = absorbance value of zero standard
2. Drawing a Standard Curve
To draw a standard curve, the absorbance value of standards as y-axis, semilogarithmic of the concentration of the standards (ppb) as x-axis.
The concentration of each sample (ppb), which can be read from the standard curve, is multiplied by the corresponding dilution factor of each sample followed, and the actual concentration of sample is obtained.