Peanut Mottle Virus (PeMoV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect PeMoV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Peanut mottle virus (PeMoV) is a plant pathogenic virus of the family Potyviridae. As with other members of this virus family, PeMoV is a flexuous filamentous virus with particles 740-750 nm long. It is transmitted by several species of aphids and by mechanical inoculation. It was first given its name in 1965 when it was isolated from peanuts (Arachis hypogaea) in Georgia, USA. This virus was found to be seed transmitted in this host.
The symptoms of this virus in peanuts appear as irregular dark islands on young leaves. The symptoms are not as clear on older leaves and thus can be easily missed even when the virus is in epidemic proportions in the field.
In infected plant cells, the virus makes characteristic potyvirus cylindrical inclusions. that are visible in the light microscope with proper staining.

Standard Curve