Intended Use
The Parkin ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Parkin protein expression profile in cells. The kit can be used for measuring the relative amounts of Parkin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Parkin.
Storage
Upon receipt, the kit should be stored at 4°C. The un-opened kit will be stable for up to 6 months from the date of shipment if stored at 4°C. Diluted Anti-Parkin Antibody and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-Conjugated Anti-Rabbit IgG Antibody and HRP-Conjugated Anti-Mouse IgG Antibody will be stable at 4°C for up to six months. The SDS Solution should be stored at room temperature or warmed up to room temperature if stored at 4°C.
Citations
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Ramsey, CP; Giasson, BI; et al. Identification and characterization of a novel endogenous murine parkin mutation. JOURNAL OF NEUROCHEMISTRY 113:402-417(2010).
Guerrero, R; Navarro, P; et al. Hyperphosphorylated tau aggregates in the cortex and hippocampus of transgenic mice with mutant human FTDP-17 Tau and lacking the PARK2 gene. ACTA NEUROPATHOLOGICA 117:159-168(2009).