Papaya Mosaic Virus - AP strain (PapMV-AP strain) ELISA Kit

Name: Papaya Mosaic Virus - AP strain (PapMV-AP strain) ELISA Kit
SKU: DEIAPV201
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect PapMV-AP Strain in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Papaya mosaic virus (PapMV) is a plant pathogenic virus in the genus Potexvirus and the family Alphaflexiviridae. PapMV is a filamentous, flexuous rod, 530 nm in length.
The virus is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The genome has been completely sequenced and is 6656 nucleotides long. It is transmitted by mechanical inoculation. No insect vector is involved and it is not transmitted in seeds.
Mature virions form banded inclusions that can be seen in leaf strips of infected plants when stained with either the Orange-Green protein stain or the Azure A nucleic acid stain.
Its principle host is papaya (Carica papaya), although in host range studies, PapMV was able to infect a few other plants including snapdragons (Antirrhinum majus). It is also reported to naturally infect Ullucus tuberosus.
PapMV was first reported in papaya in 1962 together with another virus that became known as Papaya ringspot virus (PRSV), a potyvirus. The two viruses were differentiated in 1965 and 1967 using particle lengths, serology, host range, inclusions, and aphid transmissibility.
PapMV causes mild mosaic symptoms on papaya leaves and stunting of the plant. It is known to occur in South America (Bolivia, Peru, and Venezuela) and the USA.

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Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Identification and full sequence of an isolate of Alternanthera mosaic potexvirus infecting Phlox stolonifera

ARCHIVES OF VIROLOGY

Authors: Hammond, J; Reinsel, MD; Maroon-Lango, CJ

Abstract

A potexvirus was isolated from creeping phlox (Phlox stolonifera) plants from a commercial nursery in Pennsylvania. The virus was serologically related to clover yellow mosaic virus, plantain virus X, potato virus X, and potato aucuba mosaic virus, and was most closely related to papaya mosaic virus (PapMV). The sequence of a PCR fragment obtained with potexvirus group-specific primers was distinct from that of PapMV; the coat protein (CP) gene and 3' untranslated region (UTR) were closely related to Alternanthera mosaic virus (AltMV), previously reported only from Australia. The host range was similar to that of the Australian isolate (AltMV-Au), and the phlox isolate reacted strongly with antiserum to AltMV-Au. The full sequence of the phlox isolate was more closely related to PapMV throughout the genome than to any potexvirus other than AltMV-Au, for which only the CP and 3'UTR sequences are available. The phlox isolate was therefore named AltMV-PA (for Pennsylvania), and the full 6607 nt sequence is presented(1). Additional AltMV isolates from creeping phlox (AltMV-BR and AltMV-SP) and trailing portulaca (Portulaca grandiflora; AltMV-Po) were also isolated, suggesting that AltMV may be widespread, and may have been mis-diagnosed in the past as PapMV. AltMV has the potential to spread to other ornamental crops.

In vitro virus elimination in three Andean root crops: oca (Oxalis tuberosa), ulluco (Ullucus tuberosus), and arracacha (Arracacia xanthorrhiza)

NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE

Authors: Fletcher, PJ; Fletcher, JD

Abstract

Accessions of three Andean root crops-oca (Oxalis tuber osa Molina), ulluco (Ullucus tuberosus Caldas), and arracacha (Arracacia xanthorrhiza Bancr.) were imported into quarantine in New Zealand. Using nitrocellulose membrane-ELISA, herbaceous hosts, and electron microscopy, the accessions were found to be infected with the following viruses. In oca: Arracacha vials B (AVB), Papaya mosaic vii us (PapMV), and Ullucus mild mottle virus (UMMV); in ulluco: Ullucus virus C (UVC), Ullucus mosaic virus (UMV), PapMV, and UMMV; and in arracacha: Arracacha A virus (AVA). To comply with quarantine requirements the viruses had to be eliminated. Except with one accession of oca that was infected with TMV/U, this was successfully achieved using a protocol of in vitro virus elimination that combined heat and chemical therapy. Stem nodal sections of oca and ulluco and shoot tips of arracacha were established in tissue culture on a growth medium containing 50 mg/litre ribavirin. These explants underwent 10 days of continuous heat therapy of alternating 4 h light at 35 degreesC: 4 h dark at 31 degreesC, after which time the resultant shoots were 1 cm long and ready to be excised and grown up on growth medium without ribavirin. They were grown under normal tissue culture conditions of 24 degreesC under fluorescent lights with a photoperiod of 16 h. The plantlets were then introduced to glasshouse conditions in preparation for field trials, analyses, and evaluations.