Anti-PXK (aa 163-193) polyclonal antibody

The effect of various N, P and K supply levels and their combinations on the development, yield and yield components of poppy (cv. Kek Duna) was studied in the 10(th) year of a long-term mineral fertilisation experiment set up on loamy chernozem soil with lime deposits. A correlation was sought between the mass of the poppy plants and other parameters. The soil of the growing site contained 3 % humus, 5 % CaCO3 and 20 % clay in the ploughed layer and was poorly supplied with P and Zn and moderately well with N and K. The experiment included 4Nx4Px4K=64 treatments in 2 replications, giving a total of 128 plots. The mineral fertilisers were applied in the form of calcium ammonium nitrate containing 25 % N, superphosphate (18 % P) and potassium chloride (50 % K). The groundwater was at a depth of 13-15 m and the area was liable to drought. The main results were as follows: 1. The PxK interactions were already pronounced in the early rosette phase of development, and flowering was initiated and completed 7-10 days earlier on well-supplied soil. In the dry year of 1983 a seed yield of 200 kg/ha was obtained on soil unfertilised for 10 years, while the yield was 800 kg/ha in the optimum N1P1K2 treatment. In this same treatment the capsule yield rose from 182 kg/ha to 586 kg/ha and the stalk yield from 708 kg/ha to 2084 kg/ha. 2. An optimum supply level was provided by 177 mg/kg AL-P2O5 and 232 mg/kg AL-K2O in the ploughed layer. A balanced PK supply was able to counteract the negative effect of drought to a considerable extent and even over-fertilisation did not cause a significant yield depression. Excessive N, however, led to a significant 10 % reduction in plant number. 3. The total air-dry yield amounted to 1.3 t/ha on the control plot and to 4.3 t/ha in the N1P1K2 treatment. On average the roots made up 16 %, the stalks 49 %, the capsules 15 % and the seed 20 % of the air-dry yield at harvest. 4. An improvement in the PK supplies also led to an increase in the capsule mass, the number of capsules per plant and the number of capsules per hectare. P fertilisation had a decisive influence on the yield components: the 1000-seed mass rose on average by 15 %, the number of seeds per capsule by 37 %, the seed yield per capsule by 52 % and the seed yield per plant by 106 %. 5. The individual plant production showed differences of an order of magnitude. In an 80-100 cm stand, plants measuring only 50-60 cm arc unable to satisfy their light and nutrient requirements, leading to a drastic reduction in capsule number, capsule diameter, 1000-seed mass, seed mass per capsule, seed oil % and oil and morphine base yield. The analysis of individual plants showed that poorly developed status is also indicated by low P and K concentrations or uptake.

Single-nucleotide polymorphisms (SNPs) in the UHRF gene have been shown to be associated with systemic lupus erythematosus (SLE) in European and Hong Kong Chinese, but statistically significant evidence for association has not been found in a mainland Han Chinese population. Therefore, we selected SNP rs13205210 located in UHRF1BP1 as a candidate association from our previously published genome-wide association study (GWAS) data of SLE (1,047 cases and 1,205 controls from a mainland Han Chinese population) to explore the association between the UHRF1BP1 gene and SLE. We conducted a large-scale replication study in an additional independent sample of 3,509 cases and 8,246 controls from a mainland Han Chinese population. Real-time PCR was used to determine gene expression differences in peripheral blood mononuclear cells (PBMCs) from cases and controls. As a result, we replicated the association between the UHRF1BP1 gene and SLE (rs13205210, missense, P-meta( )= 2.26E-17, odds ratio = 1.41) by a meta-analysis of our previous GWAS and this replication study involving a total of 4,556 cases and 9,451 controls. The UHRF1BP1 mRNA expression level in PBMCs was significantly decreased in patients with SLE compared with that in healthy controls. SNP rs13205210 exhibited an expression quantitative trait loci effect on the UHRF1BP1 gene in PBMCs from patients. In conclusion, this study not only suggests that the UHRF1BP1 gene was associated with SLE in a mainland Han Chinese population, but also implied that it might be a common genetic factor contributing to SLE susceptibility in multiple populations.