Potato Virus X (PVX) ELISA Kit

Name: Potato Virus X (PVX) ELISA Kit
SKU: DEIAPV8
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Host plants

Species Reactivity

Host plants

Detection Method

sELISA

Intended Use

The test can be used to detect PVX in infected potato leaves, tuber eyes, sprouts, and stems.

Contents of Kit

1. Coating Antibody: 0.25 mL. Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate.
2. Detecting Conjugate, alkaline phosphatase: 0.25 mL.
3. ELISA Plate: 5×96T. Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results.
4. Coating Buffer 10×: 6 mL
5. Sample Buffer: 11.6g
6. Conjugate Buffer 10×: 6 mL
7. PBST Buffer (Powder): 28.7g
8. Tween-20: 7.0g
9. PNP Buffer (5×): 10 mL
10. PNP Tablet: 10 Tablets

Storage

Store all buffers at 2-8°C, except for PNP tablets at -20°C under dark.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Influence of cytoplasmic heat shock protein 70 on viral infection of Nicotiana benthamiana

MOLECULAR PLANT PATHOLOGY

Authors: Chen, Zhaorong; Zhou, Tao; Wu, Xuehong; Hong, Yiguo; Fan, Zaifeng; Li, Huaifang

Abstract

The accumulation of heat shock protein 70 (Hsp70) generally occurs in plants infected with viruses. However, the effect of Hsp70 accumulation on plant viral infection and pathogenesis remains elusive. In this study, the expression of six Hsp70 genes was found to be induced by the four diverse RNA viruses, Tobacco mosaic virus, Potato virus X (PVX), Cucumber mosaic virus and Watermelon mosaic virus, in Nicotiana benthamiana. Heat treatment enhanced the accumulation and systemic infection of these viruses. Similar results were obtained for viral infection in plants heterologously expressing an Arabidopsis cytoplasmic Hsp70 through either a PVX vector or Agrobacterium infiltration. In contrast, viral infection was compromised in cytoplasmic NbHsp70c-1 gene-silenced plants. These data demonstrate that the cytoplasmic Hsp70s can enhance the infection of N. benthamiana by diverse viruses.

DETECTION OF PVY, PLRV AND PVX POTATO VIRUSES IN SOME REGIONS OF SERBIA

COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES

Authors: Ciric, Slavica; Cvetkovic, Gordana; Stojkovic, Slavisa; Gudzic, Slavisa

Abstract

During a one-year period, the testing of samples of seed potatoes from the territories of Moravica, Zlatibor, RaSka and Maeva districts of the Republic of Serbia was carried out. The presence of the three most important viruses: PVY, PLRV and PVX, was determined using serological DAS-ELISA test. Out of all tested samples, 34.62% were positive for some of these viruses. The greatest frequency was shown by PVY virus (86.36%), and significantly lower frequencies were shown by PLRV (11.62%) and PVX (2.02%) of all infected samples. PVY was detected in all districts, PLRV in all except Maeva district, and PVX was detected in the Moravica district, only. The results obtained indicate that the virus diseases could be one of the reasons for the lower potato yield in Serbia compared to Europe. This imposes the need for the application of integrated protection of potatoes.