Peanut Stunt Virus - Western Strain (PSV-WS) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect PSV-WS in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Peanut stunt virus (PSV), a member of the plant virus genus Cucumovirus, was first described in 1966 and has since been reported causing epidemics in legume crops in the Americas, Europe, Asia and Africa. The complete nucleotide sequences of the Japanese and ER strains of PSV have been determined, showing a genome organization homologous to that of cucumber mosaic virus (CMV). Soon after its first description, biological diversity among PSV strains was noticed and two strain subgroups were proposed, based on host range, particle stability and immunological relationships. These subgroups were named Eastern (E) and Western (W) according to the origin within the USA of the type strains (E and W) of each group. Most reported PSV isolates belong to the E-type, which appears to be more diverse than the W-type. Classification of PSV strains into two subgroups was further supported by competition hybridization analyses and, recently, by nucleotide sequence analysis of RNA 3, which showed identities of above 90% for subgroup I (E-type) strains and of about 80% between subgroup I and subgroup II (W-type) strains. Nevertheless, serology comparisons involving primarily Old World isolates suggest a higher diversity for PSV, as there are strains that do not fit clearly into types E (subgroup I) or W (subgroup II). This was especially the case for isolates originally classified as robinia mosaic virus.

Standard Curve