The test can be used to detect PMTV in host plants.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
General Description
Potato mop-top virus (PMTV) is a plant pathogenic virus transmitted through the vector Spongospora subterranea that affects potatoes. PMTV is a member of the genus Pomovirus. The virus, first found in Germany has now spread throughout Europe, Asia, South America, and North America. Many disease management systems have been found to be ineffective against the virus, although a combination of sanitation and vector controls seems to work well. As the name implies, the main host is potato; however, this virus also affects some common vegetable plants and weeds. Some of them include tomato, black nightshade, lambsquarters and ground cherry. The potato mop top virus' primary hosts are plants in the Solanaceae and Chenopodiaceae. Potatoes that are infected by PMTV generally show hollow necrotic spots on the inside. This virus shows different symptoms in Europe; for example, the infected plants usually show dark brown necrotic arcs that discolor the tuber's flesh (these symptoms are very similar to those caused by alfalfa mosaic virus). Interestingly, plants showing no symptoms of PMTV tend to produce larger quantities of infected tubers if they are derived from plants that have shown foliar symptoms in the previous year.
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