Class III Phosphoinositide 3-kinases ELISA Kit

Name: Class III Phosphoinositide 3-kinases ELISA Kit
SKU: DEIA-XYZ8
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Intended Use

The Class III Phosphoinositide 3-kinases ELISA Kit is a competitive (96-well) ELISA assay kit that measures Class III Phosphoinositide 3-kinases activity directly through its reaction product, PtdIns(3)P (PI(3)P).

Contents of Kit

1. PI(3)P Detection Plate, 12 x 8-strip well plate: 1 plate
2. PI Substrate, diC8, MW = 608.6 g/mol (store at -20°C): 1 vial
3. PI(3)P Standard, diC8, MW = 732.5 g/mol (store at -20°C): 1 vial
4. 5xPI(3)P Detection Buffer (5xDB): 1 bottle
5. 100 mM EDTA, pH 8: 1 vial
6. 10x TBS-T Buffer: 1 bottle
7. PI(3)P Detector (store at -20°C): 2 vials
8. Secondary Detector: 1 vial
9. TMB Solution: 1 bottle
10. 1 N H2SO4 Stop Solution: 1 bottle
11. Colored 96-well polypropylene U-bottom plate: 1 plate
12. Clear acetate sheet, 1 side adhesive: 3 sheets

Storage

2-8°C and -20°C

General Description

The Class III Phosphoinositide 3-kinases ELISA Kit is a competitive ELISA in which the signal is inversely proportional to the amount of PI(3)P produced. After the PI3-K reactions are complete, reaction products are added to the PI(3)P-coated microplate, for competitive binding to a PI(3)P detector protein. The amount of PI(3)P detector protein bound to the plate is determined through colorimetric detection.

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