Pea Enation Mosaic Virus (PEMV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect PEMV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Pea enation mosaic virus (PEMV) is a plant pathogenic virus.
The two RNAs of the disease are now categorised as two separate, mutualistic viruses: PEMV-1 is an Enamovirus, while PEMV-2 is an Umbravirus.
It is spread by green or pea aphids and affects legumes as pea, alfalfa, broadbean or sweet pea mostly in temperate regions.
Symptoms include chlorotic, translucent or necrotic lesions, malformation of leaves and stipules, and plant distortion. However, the most characteristic symptom is the formation of enations on the abaxial, i.e. downy, leaf side. Enations are derived from the cells of vascular bundles undergoing hyperplasia.

Standard Curve