PD-L1/B7-1 (Biotiny) Inhibitor Screening Assay Kit

Intended Use

The PD-L1/B7-1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the PD-L1/B7-1 interaction.

Contents of Kit

1. PD-L1, Fc fusion: 25 μg, -80°C;
2. B7-1 (CD80), Fc fusion, Biotin-labeled: 15 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x PD-L1 Assay Buffer: 50 ml, -20°C;
5. Blocking Buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (transparent bottle): 6 ml, +4°C
7. HRP chemiluminescent substrate B (brown bottle): 6 ml, +4°C;
8. White 96-well microplate: 1, +4°C .

Storage

One year from date of receipt when stored as directed.

General Description

There have been a number of studies defining a role for PD-L1 in the regulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1. Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD- 1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation, proliferation, and cytokine production, suggesting that this pathway may also serve as a target in cancer immunotherapy and inflammatory diseases.