The test can be used to detect PBRSV in infected potato leaves, tuber eyes, sprouts, and stems.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:2430.
General Description
Potato black ringspot virus (PBRSV) is a plant pathogenic virus of the family Comoviridae. A virus with particles c. 25 nm in diameter, sedimenting as three components, and with a RNA genome in two pieces. It has a wide host range, is readily transmitted by inoculation with sap and occurs in Peru. Causes systemic necrotic spotting in foliage of potato (Solanum tuberosum). The potato calico strain is associated with an intense generalized yellowing of the leaves in some cultivars, notably cv. Ticahuasi.
Standard Curve
Citations
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