PAM Knockout Cell Lysate from HeLa cell line for anti-PAM antibody specificity validation.
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The PAM knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
PAM; peptidylglycine alpha-amidating monooxygenase; peptidyl-glycine alpha-amidating monooxygenase; PAL; peptidyl alpha hydroxyglycine alpha amidating lyase; peptidylglycine alpha hydroxylating monooxygenase
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
The protein concentration was determined with BCA assay.
Store at -20°C. Avoid repeated freeze-thaw cycles.