Oat Mosaic Virus (OMV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect OMV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant tissues diluted at 1:810.

General Description

Oat mosaic virus (OMV) is a plant pathogenic virus of the family Potyviridae. A soil-borne virus with flexuous, rod-shaped particles about 600-750 nm long and 12-14 nm wide. It is mechanically transmissible but with difficulty, Host range is restricted to Avena species. It is transmitted by the fungus Polymyxa graminis, and occurs in the USA, UK, and possibly New Zealand. Oat mosaic virus causes a disease of autumn-sown oats. The field symptoms vary with the oat cultivar, virus strain, level of infestation of the soil and with environmental conditions. The mottling symptoms are most evident in the first flush of growth in the spring and tend to be masked with further growth and warmer weather. Stunting is the principal symptom seen late in the season. On heavily infested soil, yields may be reduced 25 to 50% in tolerant cultivars and most plants of very susceptible cultivars produce no grain.

Standard Curve