Nucleosome antigen is prepared from calf thymus chromatin and contains the four core histones H2A, H2B, H3 and H4 bound to DNA of about 146 base pairs in length.
Target
Nucleosome
Nature
Native
Purity
> 90% as determined by SDS-PAGE
Format
Liquid
Buffer
20 mM Tris-HCl buffer, pH 8.0
Preservative
None
Storage
Store at -80°C
Ship
Dry ice
Introduction
A nucleosome is the basic structural unit of DNA packaging in eukaryotes. The structure of a nucleosome consists of a segment of DNA wound around eight histone proteins and resembles thread wrapped around a spool. The nucleosome is the fundamental subunit of chromatin. Each nucleosome is composed of a little less than two turns of DNA wrapped around a set of eight proteins called histones, which are known as a histone octamer. Each histone octamer is composed of two copies each of the histone proteins H2A, H2B, H3, and H4.
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