Nitrotyrosine ELISA kit

Sample

Plasma, biological samples

Species Reactivity

Universal

Detection Method

sELISA

Intended Use

The Nitrotyrosine ELISA kit is to be used for the in vitro quantitative determination of nitrotyrosine in plasma and other biological samples. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

Contents of Kit

Storage

1.Upon receipt, store individual components at 2 - 8°C. Do not freeze.
2. Do not use components beyond the expiration date printed on the kit label.
3.The standard, tracer in lyophilized form and streptavidin-peroxidase in concentrated solution are stable until the expiration date indicated on the kit label, if stored at 2 - 8°C.
4.The exact amount of the standard is indicated on the label of the vial and the Certificate of Analysis .
5.The standard is single use. After reconstitution the standard cannot be stored.
6.Once reconstituted the tracer are stable for 1 month if stored at 2 - 8°C.
7.The streptavidin-peroxidase can only be stored in concentrated solution and is not stable when stored diluted.
8.Upon receipt, foil pouch around the plate should be vacuum-sealed and unpunctured. Any irregularities to aforementioned conditions may influence plate performance in the assay.
9.Return unused strips immediately to the foil pouch containing the desiccant pack and reseal along the entire edge of the zip-seal. Quality guaranteed for 1 month if stored at 2 - 8°C.

Detection Range

2-1500 nM

Detection Limit

2 nM

General Description

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NO_X is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NO_X levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.