Intended Use
The eNOS (Phospho-Ser615) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect eNOS protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated eNOS in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eNOS phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate, 2 Plates
2. 10× TBS, 24 ml (10×), Clear
3. Quenching Buffer, 24 ml (1×), Clear
4. Blocking Buffer, 50 ml (1×), Clear
5. 15× Wash Buffer, 50 ml (15×), Clear
6. 100× Anti-eNOS Antibody (Phospho-Ser615), 60 μl (100×), Red
7. 100× Anti-eNOS Antibody (Rabbit Polyclonal), 60 μl (100×), Purple
8. 100× Anti-GAPDH Antibody (Mouse Monoclonal), 60 μl (100×), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 ml (1×), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 ml (1×), Glass
12. Primary Antibody Diluent, 12 ml (1×), Clear
13. Ready-to-Use Substrate, 12 ml (1×), Brown
14. Stop Solution, 12 ml (1×), Clear
15. Crystal Violet Solution, 12 ml (1×), Glass
16. SDS Solution, 24 ml (1×), Clear
17. Adhesive Plate Sealers, 2 Seals
Storage
Upon receipt, the kit should be stored at 4°C. The un-opened kit will be stable for up to 6 months from the date of shipment if stored at 4°C. Diluted Anti-eNOS (Phospho-Ser615) Antibody, Anti-eNOS Antibody and diluted Anti- GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP- Conjugated Anti-Rabbit IgG Antibody and HRP-Conjugated Anti-Mouse IgG Antibody will be stable at 4°C for up to six months. The SDS Solution should be stored at room temperature or warmed up to room temperature if stored at 4°C.
COA
Publication 
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