Conjugation is determined at pH 8.5 by measuring NIP absorbance at 430nm, using the extinction coefficient of NIP= 5.0 x 10E+03. The conjugation ratio is given as a molar ratio of NIP to OVAL assuming the molecular weight of OVAL is 45,000. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water.
Preservative
None
Storage
2 to 8 °C
Ship
Ambient
Introduction
4-Hydroxy-3-iodo-5- nitrophenylacetyl hapten is conjugated to OVAL protein through lysine by amide bonds.
Citations
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References
Towards permanent hydrophilic PVDF membranes. Amphiphilic PVDF-b-PEG-b-PVDF triblock copolymer as membrane additive
Poly(vinylidene fluoride) (PVDF) ultrafiltration membranes were prepared by non-solvent-induced phase separation (NIPS) using a blend of a new amphiphilic PVDF-based triblock copolymer (PVDF-b-PEG-b-PVDF) and high molar mass PVDF. During the phase inversion step, the triblock copolymer acts both as a pore forming and surface modifying agent. Thanks to the presence of the short PVDF blocks in the triblock copolymer, the hydrophilic PEG segment is fixed in the PVDF matrix, thus reducing the gradual loss of hydrophilic additive by leaching of the triblock copolymer out of the membrane matrix. The PVDF-b-PEG-b-PVDF triblock copolymer additive improved the surface hydrophilicity and significantly increased the pure water flux and permeability of the PVDF membrane. The membrane composition was optimized in terms of additive concentration and compared to membranes prepared with an equivalent amount of commercial PEG of similar molar mass. Pure water filtration tests and contact angle measurements suggested that addition of small amounts of the triblock copolymer additive (2-5% w/w) has a strong impact on the performance and hydrophilic characteristic of the PVDF membranes. The control tests showed that less than 30% w/w of the additive is lost after 9 months, while most commercial PEG (59% w/w) leached out of the membrane matrix in only two months.
Distality for the Asymptotic Couple of the Field of Logarithmic Transseries
We show that the theory T-log of the asymptotic couple of the field of logarithmic transseries is distal. As distal theories are NIP (have the non-independence property), this provides a new proof that T-log is NIP.
COA
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