Sample
cell culture supernatants, tissues homogenates, serum, plasma
Species Reactivity
Mouse; Rat
Detection Method
Sandwich ELISA
Intended Use
For the quantitative determination of mouse and rat Repulsive Guidance Molecule A (RGM-A) concentrations in cell culture supernates, tissue homogenates, serum, and plasma.
Contents of Kit
1. Mouse/Rat RGM-A Microplate: 1 x 96 wells
2. Mouse/Rat RGM-A Conjugate: 1 x 12 mL
3. Mouse/Rat RGM-A Standard: 25 ng
4. Mouse/Rat RGM-A Control: 1 vial
5. Assay Diluent: 1 x 12 mL
6. Calibrator Diluent: 1 x 21 mL
7. Wash Buffer Concentrate(25X): 1 x 50 mL
8. Color Reagent A: 1 x 12 mL
9. Color Reagent B: 1 x 12 mL
10. Stop Solution: 1 x 23 mL
Storage
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Precision
Intra-assay Precision:
2.4-5.4%
Inter-assay Precision:
5.3-7.6%
Detection Range
0.004-0.019 ng/mL
Citations
Publication ()
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