Mouse MIP-1β ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant MIP-1β in a sandwich ELISA format. Using the ELISA protocol described below, this kit provides sufficient reagents to assay MIP-1β in approximately 1500 ELISA plate wells.
Contents of Kit
1. Capture Antibody 2. Detection Antibody 3. Standard 4. StreptAvidin-HRP 5. TMB Liquid Substrate "Ready to Use"
Storage
See "Reagent Preparation"
General Description
Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.
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