Methyltestosterone ELISA kit

Sample

Serum, plasma and other biological fluids

Species Reactivity

Universal

Detection Method

sELISA

Intended Use

This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MET in general serum, plasma and other biological fluids.

Contents of Kit

1. Pre-coated, ready to use 96-well strip plate:
2. Plate sealer for 96 wells: 2
3. Standard: 2
4. Diluent Buffer: 1 x 45 mL
5. Detection Reagent A: 1 x 120 μL
6. Detection Reagent B: 1 x 120 μL
7. TMB Substrate: 1 x 9 mL
8. Stop Solution: 1 x 6 mL
9. Wash Buffer (30x concentrate): 1 x 20 ml

Storage

1. For unopened kits: All the reagents should be kept according to labels on the vials. The TMB Substrate, Wash Buffer (30× concentrate)and the Stop Solution should be stored at 4°C C upon receipt while the others should be at -20°C.
2. For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

Note:
For the expiration date of the kit, please refer to the label on the kit box. All components are stable until this date. It is highly recommended to use the remaining reagents within 1 month of opening.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MET were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MET were tested on 3 different plates, 8 replicates in
each plate.
CV (%) = SD/mean × 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Detection Range

78.125-5000 pg/mL. The concentrations used for creating the standard curve were 5000 pg/mL, 2500 pg/mL, 1250 pg/mL, 625 pg/mL, 312.5 pg/mL, 156.25 pg/mL, 78.125 pg/mL.

Sensitivity

The minimum detectable dose of MET is typically less than 31.3 pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.