Metanephrine(plasma) ELISA Kit

Sample

Plasma

Species Reactivity

Human

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for measurement of free Metanephrine in plasma.

Contents of Kit

1. Reaction Tubes: 2×50 tubes; ready for use
2. Adhesive Foil: 1×4; ready for use
3. Wash Buffer Concentrate: 1×20 mL; Concentrate. Dilute content with dist. water to a final volume of 1000 mL
4. Enzyme Conjugate: 1×12 mL; ready for use, anti-rabbit IgG conjugated with peroxidase
5. Substrate: 1×12 mL; ready for use, containing a solution of tetramethylbenzidine (TMB)
6. Stop Solution: 1×12 mL; ready for use, containing 0.25 M H₂SO₄
7. Adrenaline-Metanephrine Microtiter Strips: 1×96 wells; 12 strips, 8 wells each, break apart, pre-coated, blue coloured
8. Metanephrine Antiserum: 1×6 mL; from rabbit, ready for use, blue coloured, blue screw cap
9. Assay Buffer: 1×12 mL; ready for use
10. Equalizing Reagent: 2×10 mL; lyophilized
11. Standard A: 1×12 mL; ready for use
12. Standard B: 1×4 mL; ready for use
13. Standard C: 1×4 mL; ready for use
14. Standard D: 1×4 mL; ready for use
15. Standard E: 1×4 mL; ready for use
16. Standard F: 1×4 mL; ready for use
17. Acylation Concentrate: 1×1.5 mL; concentrated
18. Control 1: 1×4 mL; ready for use
19. Control 2: 1×4 mL; ready for use

Storage

Store the unopened reagents at 2-8°C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened, the reagents are stable for 1 month when stored at 2-8°C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant again.

Precision

Sensitivity

General Description

Metanephrine is a metabolite of epinephrine created by action of catechol-O-methyl transferase on epinephrine. An article in the Journal of the American Medical Association, 2002, indicated that the measurement of plasma free metanephrines is the best tool in the diagnosis of pheochromocytoma, an adrenal medullary neoplasm.

Standard Curve