Maize strip virus (MSpV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect MSpV in host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

General Description

Maize stripe virus (MSpV) is a pathogenic plant virus. A virus with fine filamentous particles 3 nm in diameter and of undetermined length. The particles sediment as four major components, contain a single species of coat protein and five species of single-stranded RNA. Large amounts of a disease-associated non-particle or 'non-capsid' (NC) protein are found in infected tissue. The only known vector is the delphacid planthopper, Peregrinus maidis, which transmits the virus in the persistent propagative manner and transovarially. It is not transmitted by sap inoculation. The virus infects only a few species of Gramineae. It is found throughout the tropics where it occasionally causes serious losses. In maize (Zea mays), the virus causes severe chlorotic striping and streaking with frequent apical bending . Infected plants are stunted and produce little or no grain.