Maize streak virus (MSV) ELISA Kit

Name: Maize streak virus (MSV) ELISA Kit
SKU: DEIAPV187
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect MSV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 2430.

General Description

Maize streak virus causes a plant disease, known as maize streak disease (MSD) in its major host. It is an insect-transmitted maize pathogen in the genus Mastrevirus of the family Geminiviridae that is endemic in sub-Saharan Africa and neighbouring Indian Ocean island territories such as Madagascar, Mauritius and La Reunion. The A-strain of MSV (MSV-A) causes sporadic maize streak disease epidemics throughout the maize growing regions of Africa. MSV was first described by the South African entomologist Claude Fuller who referred to it in a 1901 report as "mealie variegation". MSV is mainly transmitted by Cicadulina mbila Naude, but other leafhopper species, such as C. storeyi, C. arachidis and C. dabrowski, are also able to transmit the virus.
Typical of all mastreviruses, MSV's circular, ~2.7-Kb monopartite single-stranded (ss) DNA genome encodes only four proteins. Bidirectional transcription from a long intergenic region (LIR) results in the virion-sense expression of a movement protein (MP) and a coat protein (CP), and the complementary-sense expression of the replication-associated proteins, Rep and RepA. Whereas the MP and CP are involved in virus movement and encapsidation, Rep is an essential initiator of virus replication, and RepA is a regulator of host and viral gene transcription. Due to genome size restrictions, MSV usurps host DNA replication and double-stranded DNA break repair proteins to replicate its genome via, respectively, rolling-circle and recombination-dependent mechanisms.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

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References

Evaluation of arising exposure of ionizing radiation from computed tomography and the associated health concerns

JOURNAL OF RADIATION RESEARCH AND APPLIED SCIENCES

Authors: Abuelhia, Elfatih; Alghamdi, Ali

Abstract

Computed tomography (CT) scans involve exposure to ionizing radiation, which can present unavoidable risks. CT is one of the largest contributors to man-made radiation dose in medical populations. Despite the clinical benefits and the increase of CT imaging usage, the concern of arising exposure of ionizing radiation associated with CT imaging may pose significant health risks. This paper investigates the arising exposure of ionizing radiation from CT and associated radiation dose in the study area. The exposure of ionizing radiation from CT scans was assessed in four hospitals using active detection methods. CT scan operators were exposed to an annual effective dose (mean +/- SD) ranged from 2.83 +/- 0.4 mSv/y to 12 +/- 2.4 mSv/y. The public was exposed to annual effective dose ranged from 0.40 +/- 0.04 mSv/y to 0.87 +/- 0.04 mSv/y. Patients were exposed to minimum effective dose of 1.03 mSv (head scan) and maximum effective dose of 24.45 mSv (abdomen pelvis). The results show the occupational annual effective dose and the public annual effective dose were within the ICRP recommended dose limits. Its first kind of study in this area and the data obtained emphasizes the importance of applying strategies to optimize the radiation dose from CT scans.

Conversion factors for effective dose and organ doses with the air kerma area product in patients undergoing percutaneous transhepatic biliary drainage and trans arterial chemoembolization

PHYSICA MEDICA-EUROPEAN JOURNAL OF MEDICAL PHYSICS

Authors: Brambilla, M.; Cannillo, B.; Guzzardi, G.; D'Alessio, Andrea; Galbiati, Andrea; Matheoud, Roberta; Carriero, A.

Abstract

Conversion factors used to estimate effective (E) and organ doses (H-T) from air Kerma area product (KAP) are required to estimate population doses in percutaneous transhepatic biliary drainage (PTBD) and trans arterial chemoembolization (TACE) interventional procedures. In this study, E and H-T for ten critical organs/tissues, were derived in 64 PTBD and 48 TACE procedures and in 14,540 irradiation events from dosimetric, technical and geometrical information included in the radiation dose structured report using the PCXMC Monte Carlo model, and the ICRP 103 organ weighting factors. Conversion factors of: 0.13; 0.19; 0.26 and 0.32 mSv Gy (-1) cm(-2) were established for irradiation events characterized by a Cu filtration of 0.0; 0.1; 0.4 and 0.9 mm, respectively. While a single coefficient of conversion is not able to provide estimates of E with enough accuracy, a high agreement is obtained between E estimated through Monte Carlo methods and E estimated through E/KAP conversion factors accounting separately for the different modes of fluoroscopy and the fluorography component of the procedures. An algorithm for the estimation of effective and organ doses from KAP has been established in biliary procedures which considers the Cu filtration in the X-ray irradiation events. A similar algorithm could be easily extended to other interventional procedures and incorporated in radiation dose monitoring systems to provide dosimetric estimates automatically with enough accuracy to assess population doses.